Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1M Tris-HCl, pH 9.0 Solution, 20L Bag, Sterile. 4 Pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Kd Medical Inc TBE (TRIS-BORATE EDTA), 10X
Tris Borate EDTA is used for polyacrylamide and agarose gel electrophoresis. 10X TBE is concentrated and ready to be diluted to a 1X working solution. One 500ml bottle.
Sigma Aldrich Fine Chemicals Biosciences TRIS 3 HYDROXYPROPYLTRIAZOLY1G
Tris(3-hydroxypropyltriazolylmethyl)amine, 1g
eMolecules 850568-79-5 | 5-Boc-amino-2-hydroxymethylphenylboronic acid, dehydrate | Combi-Blocks | MFCD06659853 | 249.070 | C12H16BNO4 | 98.000 | CC(C)(C)OC(=O)Nc1ccc2COB(O)c2c1 | 1g | 117521248
5-Boc-amino-2-hydroxymethylphenylboronic acid, dehydrate | Combi-Blocks | 850568-79-5 | MFCD06659853 | 249.070 | C12H16BNO4 | 98.000 | CC(C)(C)OC(=O)Nc1ccc2COB(O)c2c1 | 1g | 117521248
Boston Bioproducts Inc Sodium Phosphate Monobasic (3 M) - 2x1L
Bioprocess Grade - Sodium Phosphate Monobasic (3 M) - 2x1L (For Research Use and Further Manufacturing Only)
Alkali Scientific SSC Buffer, 20X Solution, 4 L
SSC Buffer 20X Solution (4 L) is a large-format, ready-to-use buffer solution for high-throughput DNA hybridization experiments. This 20X concentration solution is essential for preparing a stable environment that supports the binding of nucleic acid probes to their complementary targets in hybridization protocols. SSC Buffer is commonly used in Southern and Northern blotting, as well as in situ hybridization and other DNA/RNA detection methods. The 4L volume is designed for laboratories conducting large-scale hybridization experiments, providing the necessary buffer for high-volume workflows while maintaining consistent ionic strength to ensure efficient and reliable hybridization.
Gold Biotechnology Inc Tris HCl 500 g
Tris hydrochloride is a reagent that is used for the stabilization of buffer formulations and electrophoresis of biological molecules
Biotang Inc HEPES Lysis Buffer with Triton (2X), 125 ml
HEPES Lysis Buffer with Triton (2X), 125 ml.
Biotang Inc BioAcryl-P (30%) Bis-Acrylamide 29:1, 1000 ml
BioAcryl-P (30%) Bis-Acrylamide 29:1, 1000 ml.
Bioworld TT - SDS Running Buffer (Anode) (10X), 1 L
Product DescriptionTris-Tricine (TT)-SDS running buffer has a 10X concentration and a pH of 8.9. It contains 1M Tris-base, 1M Tricine and 1% SDS.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.