Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
eMolecules N-(3-Indolylacetyl)glycine | 13113-08-1 | MFCD00079392 | 250mg
Toronto Research Chemicals | N-(3-Indolylacetyl)glycine | 250mg | 601597193 | I628005 | | 13113-08-1 | MFCD00079392 | 232.239 | C12H12N2O3
If you are unable to find the chemical you are looking for, make sure you are logged into your fishersci.com account and click on the following link:
eMolecules Building Block Tool
Hampton Research HR2-817/1.0 M ADA pH 6.5 - 100 ml
HR2-817/1.0 M ADA pH 6.5 - 100 ml. Please note items from this supplier are non-returnable.
Crescent Chemical Co Inc MORPHOLINOPROPANE SULFONICACID
Morpholinopropane sulfonic acid analytical grade pKa 20 = 7.2. Buffering substance for biochemistry, molecular and cell biology. Buffer component for isoelectric focussing and SDS PAGE. Substitute for HCO3-/CO2-buffer in cell culture for muscle studies. Size - 500G Sto
Research Products International Corp POPSO 100 G
CAS Number: 68189-43-5
Molecular Formula: C10H22N2O8S2
Molecular Weight: 362.4
Alkali Scientific CellPro™ 1M HEPES pH 7.5 (Adjusted with KOH) 1000mL
CellPro™ 1M HEPES pH 7.5 (Adjusted with KOH) 1000mL
FUJIFILM BIOSCIENCES INC RPMI Medium 1640 1X HEPES
RPMI Medium 1640 1X HEPES is designed for growth of humanLeukemia cells in either monolayers or suspension and is useful for a wide variety of suspension and monolayer cultures as well as for hybridomas
Teknova 1M HEPES, pH 7.5, 200L Bag, Sterile.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Teknova HBS-EP Buffer (10mM Hepes, 150mM NaCl, 3mM EDTA, 0.005% Tween-20) 200mL, Sterile. 10 pack.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Sigma Aldrich Fine Chemicals Biosciences HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture | 7365-45-9 | MFCD00006158 | 1kg
HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culturePurity: | MW: 238.3 | 7365-45-9 | MFCD00006158 | 1kg
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-1000.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 1 Kilogram
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
Boston Bioproducts Inc Succinate Buffer (0.2 M, pH 5.5) - 500ml
Succinate Buffer (0 2 M pH 5 5) - 500ml (For Research Use and Further Manufacturing Only)
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.5, 500 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :pKa (25 °C) :7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.5
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 7.0, 100 mL
Synonym:N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications. The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :Useful pH range :6.4 - 7.8pKa (25 °C) :7.1 (Free acid)Warning:For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.
Research Products International Corp Mops Sodium Salt, 100G, 71119-22-7, MFCD00064350
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.