Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Gold Biotechnology Inc HEPPSO 100 g
HEPPSO is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 7.1 – 8.5. It is commonly used as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPPSO displays no toxicity for crustaceans with which it has been studied and has been deemed suitable for toxicological studies. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. HEPPSO forms a complex with Cu(II) ions so stability constants and concentrations should be taken into consideration when using this buffer in a solution containing copper ions. Though HEPPSO binds to coppers ions, it is still suitable for use with the bicinchoninic acid (BCA) assay. Diminished protein-induced color development can be accounted for or even eliminated by precipitating protein prior to reaction with bicinchoninic acid.
Kd Medical Inc TRIS BUFFER, 2M, PH 7.5, 500ML
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. Tris Solution, 2M, provides a convenient stock solution for preparing Tris buffers> One 500 ml bottle.
Kd Medical Inc MOPS BUFFER 10X, 1L
10 x MOPS Buffer is intended for use at 1x as the electrophoresis and gel running buffer during the separation of RNA on denaturing formaldehyde/agarose gels. One 1 liter bottle.
Kd Medical Inc PBS, 10X, PH 7.4, 4 LITER
10X buffer solution ready for dilution to a 1X PBS working solution commonly used in biological research. Used as a wash buffer in several research applications. One 4 L cubitainer.
Kd Medical Inc DPBS, 1X, W/O CA AND MG, 1L
Dulbecco's Phosphate Buffered Saline are recommended for use in various procedures used in biological and molecular research. Solution is without Calcium and Magnesium. One 1 Liter Bottle.
Kd Medical Inc TBS, 20X, PH7.4, 2000ML
20X Tris Buffer Saline is ready for dilution to a 1X working solution. Used in various research applications. One 2 Liter cubitainer.
Kd Medical Inc PBS 20X Solution, PH 7.4, 1L
Phosphate Buffered Saline in a 20X concentrate for use as a wash buffer in immuno-labeling techniques. pH 7.4 One 1 Liter bottle.
![Research Products International Corp MOPSO [3-(N-Morpholino)-2-hydroxypropanesulfonic acid], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92060-25.0.JPG-250.jpg)
Research Products International Corp MOPSO [3-(N-Morpholino)-2-hydroxypropanesulfonic acid], 25 Grams
- Substance Name: 3-(N-Morpholino)2-hydroxypropanesulfonic acid
- CAS Number: 68399-77-9
- Molecular Formula: C7H15NO5S
- Molecular Weight: 225.3
Quality Biological Inc PBS pH 7.4 without Calcium and Magnesium, 20 Liters
PBS (phosphate buffered saline) ¿ Balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. ¿ Endotoxin and Mycoplasma tested ¿ Applications: immunological applications, such as Western Blotting. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ pH: 7.3 ¿ 7.5 ¿ Osmolality: 300¿5% mOsm/Kg H2O ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Chem-Impex International, Inc. Piperazine-N,N'-bis(2-ethanesulfonic acid) | 5625-37-6 | MFCD00006159 | 100G
Piperazine-N,N'-bis(2-ethanesulfonic acid), 5625-37-6, MFCD00006159, 100G
Research Products International Corp PIPES Sodium Salt 250 G
pKa (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5